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Caspase-5 Substrate WEHD-AFC biochemical product blog

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Posted on 2016-02-03 13:32:14 by mybiosource_staff
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Tags: Biochemical; Caspase-5 Substrate WEHD-AFC; Caspase-5 Substrate WEHD-AFC biochemical;
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The Caspase-5 Substrate WEHD-AFC n/a (Catalog #MBS842398) is a Biochemical and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below:
Ac-Trp-Glu -His-Asp-A FC.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Background: Ready-to-use fluorometric substrate for caspase-1,-4,-5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate WEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer and 2X Reaction Buffer for caspase assays are also available separately.

Caspase-5 Substrate WEHD-AFC n/a

Structure Available?: No
Salt Form: No
Cell-Permeable?: No
MDL Number: MFCD01862607
PubChem CID: 44135104. SMILES: CC(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CCC(=O)O)C(=O)NC(CC3=CN=CN3)C(=O)NC(CC(=O)O)C(=O)NC4=CC5=C(C=C4)C(=CC(=O)O5)C(F)(F)F
InChi: InChI=1S/C38H37F3N8O11/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54)
InChi Key: NEGOLAGPKZGIKJ-UHFFFAOYSA-N
Handling: Protect from light and moisture.
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